What Do You Need to Mix Semaglutide?
Before you start mixing your semaglutide, you need to gather the right equipment. It's important to work in a sterile manner to avoid contamination.
Required Equipment:
- Semaglutide vial - Lyophilized peptide in powder form
- Bacteriostatic water (BAC water) - Sterile water with 0.9% benzyl alcohol
- Insulin syringes - 1ml syringes with fine needle (29-31G)
- Alcohol swabs - For sterilizing the rubber stopper
Step 1: Preparation
Wash your hands thoroughly with soap and water. Wipe down the work surface with alcohol. Gather all accessories and allow the semaglutide vial to reach room temperature if it has been stored in the refrigerator.
Step 2: Sterilize the Rubber Stopper
Wipe the rubber stopper on both the semaglutide vial and the BAC water bottle with an alcohol swab. Let it dry for 10-15 seconds before proceeding.
Step 3: Draw Up BAC Water
Use an insulin syringe to draw up the desired amount of bacteriostatic water. For a 5mg semaglutide vial, we recommend 2ml of BAC water, which provides a concentration of 2.5mg/ml (250mcg per 0.1ml).
Calculate the Correct Amount:
- 5mg semaglutide + 2ml BAC = 250mcg per 0.1ml
- 5mg semaglutide + 2.5ml BAC = 200mcg per 0.1ml
- 10mg semaglutide + 2ml BAC = 500mcg per 0.1ml
Step 4: Inject the Water into the Vial
This is the most important step! Insert the needle through the rubber stopper and aim it at the glass wall inside the vial. Slowly push the water out so that it runs down the glass wall to the powder.
NEVER SHAKE the vial! Peptides are sensitive proteins that can be destroyed by vigorous shaking.
Step 5: Allow the Peptide to Dissolve
Gently swirl the vial between your fingers to help the powder dissolve. You can also let the vial sit in the refrigerator for 15-30 minutes - the peptide often dissolves on its own.
The liquid should become completely clear and transparent. If it is cloudy or has particles, do not use it.
Step 6: Storage After Mixing
Once semaglutide is mixed, it must be stored in the refrigerator (2-8°C). Mixed semaglutide lasts for about 28-30 days if stored correctly.
Storage Tips:
- Always store in the refrigerator
- Protect from direct light
- Write the mixing date on the vial
- Use within 30 days
Common Mistakes to Avoid
- Shaking the vial - Destroys the peptide
- Injecting water directly onto the powder - Can damage the molecules
- Using tap water - Must be sterile BAC water
- Storing at room temperature - Drastically reduces shelf life
Summary
Mixing semaglutide is simple if you follow the correct technique. Remember: work sterilely, inject the water along the glass wall, never shake, and store in the refrigerator. With these steps, you will have a safe and effective peptide solution.