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What do you need?
📦 Checklist
- Semaglutide — Lyophilized (freeze-dried) powder in a vial
- Bacteriostatic Water (BAC Water) — Sterile water with 0.9% benzyl alcohol
- Insulin Syringes — 1ml (100 units) with a fine needle
- Alcohol Swabs — For cleaning
Step 1: Prepare the Workspace
- Wash your hands thoroughly with soap for at least 20 seconds
- Wipe down the workspace with alcohol or disinfectant
- Gather all materials so you don't need to move during the process
- Let everything reach room temperature if it has been refrigerated (reduces condensation)
Step 2: Clean Rubber Stoppers
Use an alcohol swab to wipe the rubber stoppers on both vials. Allow to air dry for 30 seconds before proceeding.
Step 3: Calculate Water Amount
The amount of BAC water determines the concentration. Example for 5mg Semaglutide:
| BAC Water | Concentration | 0.25mg = |
|---|---|---|
| 2.0 ml | 2.5 mg/ml | 10 units ✓ |
| 2.5 ml | 2.0 mg/ml | 12.5 units |
| 5.0 ml | 1.0 mg/ml | 25 units |
💡 Recommendation
Use 2ml BAC water for the simplest dosing — even tens on the syringe.
Step 4: Draw Up BAC Water
- Remove the needle cap from the insulin syringe
- Draw up some air — the same amount as the water you will take
- Insert the needle through the rubber stopper on the BAC vial
- Inject the air — creates pressure for easier withdrawal
- Turn the vial upside down
- Draw up the desired amount of BAC water (e.g., 2ml = 200 units)
- Remove the syringe carefully
Step 5: Add Water to Semaglutide
🚨 CRITICAL STEP
NEVER inject the water directly onto the powder! This can damage the peptide molecules and permanently reduce effectiveness.
- Insert the needle through the rubber stopper of the Semaglutide vial
- Aim the needle tip at the glass wall — NOT at the powder
- Slowly inject the water so it runs down the wall
- Remove the syringe carefully
Step 6: Mix Gently
- Hold the vial in your hand
- Gently swirl in circular motions
- NEVER SHAKE — this destroys the peptides
- Continue until all powder is dissolved
- Check — the solution should be clear and colorless
⏱️ Time
It may take 2-5 minutes to fully dissolve the powder. Be patient! If there are particles or cloudiness after 5 minutes of gentle mixing, discard and start over.
Step 7: Storage After Mixing
✓ Storage Instructions
- Return the vial to the refrigerator (2-8°C)
- Use within 28-30 days
- Label the date on the vial so you know when it was mixed
- Store in a dark place — peptides are light-sensitive
Dosage Calculation After Mixing
With 5mg Semaglutide in 2ml BAC water (concentration: 2.5 mg/ml):
Common Mistakes to Avoid
❌ Mistake 1: Injecting Water Directly onto the Powder
This denatures (destroys) the peptide. Always inject along the glass wall.
❌ Mistake 2: Shaking the Vial
Shaking creates air bubbles and can destroy the molecular structure. Gently swirl instead.
❌ Mistake 3: Using Regular Water
Only use bacteriostatic water. Sterile water is also acceptable but has a shorter shelf life (7-10 days).
❌ Mistake 4: Incorrect Storage
Reconstituted peptide MUST be stored in the refrigerator and protected from light.
FAQ: Frequently Asked Questions
Can I use sterile water instead of BAC?
Yes, but the shelf life will be shorter (7-10 days instead of 28-30). BAC water is recommended.
How do I know if the peptide has been damaged?
Signs: cloudy solution, visible particles, color change, or significantly reduced effect.
Can I reuse the syringe?
No, always use a new sterile syringe to avoid contamination.
Summary
✓ Mixing in 5 Steps
- Prepare and clean
- Draw up the correct amount of BAC water
- Add along the glass wall
- Gently swirl — never shake
- Store in the refrigerator
With the right technique, you will achieve a stable solution that lasts up to 30 days.
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